8th October 2007
Tuesday, October 09, 2007
Shall i drop the training course? If you dropped then you will get nothing at the end.
Emotional swing is not a problem initially, for the reason of unknown, the talking intonation is different during the group and one-on-one discussion, sometimes demanding and not according to our ability.., however we still have to apply the surviving golden rule, "just follow, it's for your own good..." Actually being treated like this is good, at least got someone care for you.., shaping you to be a better person... Haha...
This is good.., we can use this motivate ourself...
No Pain No Gain… Accept the Pain, and the Future will be Fruitful…
Don't feel the work you are doing is pain, because there will be always a reason for that pain or work.
So face the pain, for the pain you face, there will be definitely happiness a head.
Labels: 3517, Lab Rotation, Meaningful, Teoh Pak Guan
posted by teopag @ 12:03 AM,
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21st April 2007
Saturday, April 21, 2007
Opps.., didn't go to collect the cell lysate for 72 hours yesterday evening. Straightaway went back to study for exam after lunch....:( Hopefully it will not affected the results later. Anyway, the 96 hours have to be collected in the late afternoon today....
Today exam had 6 papers, i only have confident for 4 of them. We have to explain for the answers on true-and-false questions, luckily i know most of the answers. Two chapters read on this morning was not really understand and study well, so just wrote whatever i know.., don't know got mercy points or not. opefully can pass lah...
Today is Kean Jin and Sun Li geh ROM, so sorry cannot attend.., got exam ma... ;p
Wish them "forever bath in love river" and "forever bind with same heart". Congratulations!!
Labels: Exam, Lab Rotation, Laboratory, Marriage
posted by teopag @ 12:38 PM,
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16th April 2007
Monday, April 16, 2007
Ahhh..., that day geh experiments not successful, the control didn't show translation in the cells, probably i didn't add RNase inhibitors and not mixing the RNA and cells properly. Thought of repeating today, but then cell number not enough. Luckily Po Jiang give me the cells and i can do tomorrow.
Today i presented my work so far during lab meeting, it's my first time to present work in Mandarin.., feel a bit weird. And of course i was nervous worrying what i explained was wrong and kena tembak... Haha, no la.., so far so good.
Happy birdday to Boon Chee for yesterday...
Happy birthday to Seng Chuan today.
Labels: Lab Rotation, Laboratory
posted by teopag @ 10:10 PM,
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14th April 2007
Saturday, April 14, 2007
Yesterday i carried out the experiment by myself, from in vitro transcription synthesising subgenomic replicon RNA to infect the cells through electroporation. I was a bit chicken hand duck leg (clumsy) during the tissue culture part as i was not confident, so many procedures need to be carried out properly. Finally i made it! So now need to monitor the expression level of reporter gene indicating the replication of introduced RNA in the cells which will eventually contribute to the stable clones establishment. So need to monitor for 5 days to see the replication and two weeks for the stable clones. Orz...
Today is Saturday, but it's a working day...to replace the holiday on last Friday. Good Friday is not a public holiday here, because last Thursday is Qing Ming. Qing Ming (fall on 5th of April every year) is the day for the Chinese to visit the graveyard showing their appreciation and gratitude for the contribution of their ancestors and grandparents. It is a good culture to cultivate the morality among the young generation especially on appreciation and gratefulness of the old generations. It would be a rainy day, and the sky will appeared moody yet clear and bright, that's why it's called Qing (clear) Ming (bright)...
Today got class, because last Friday's class was postponed because of "Good Friday"... Next Saturday is the mid term exam for Cellular and Molecular Immunology. Alamak, semua belum sentuh lagi...
Labels: Lab Rotation, Laboratory, Qing Ming
posted by teopag @ 1:34 AM,
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Finally i told her...
Monday, April 09, 2007
Today i told my PI Dr. Wu Huey-Nan that i would like to stay in the lab after my lab rotation, she agreed... :) She shared with me some of her feeling and experience in dengue research, haha we are all human... Still having some ambiguity in the findings, so need to find out the niche area and get the present work done.
Frank was trying to install Windows XP (downgrading from Windows Vista), it wasn't successfull due to some settings in BIOS (merely speculation)... so he suggested me to use other softwares which are actually solving my problem... so i just have to change the software which i was using all the while and changed to others which is supported by Vista. Hoho...
Thanks a lot!
Oh ya.., Happy Birthday to Catherine... the zabor la...
This is taken by Prafulla, she bought a new camera.., of course digital la.., now where got people want to buy camera using film one.., unless you are one of those professionals...
Labels: Lab Rotation, laptop
posted by teopag @ 11:18 PM,
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30th March 2007
Friday, March 30, 2007
I wish to finish the cloning life for lab rotation...and proceed to tissue culture to test the constructs.. it was interesting doing such experiment... and i don't have to kill any mice for experimental purposes...
Doing cloning is fast here, we can send for sequencing and get the results in the same day or the next day. and mutants can be created with commercial kits...
Miranda sent me curry powder she bought from India, thanks a lot...
Today i found a journal on Faye Wong:
Anthony Fung and Michael Curtin (2002) The anomalies of being Faye (Wong): Gender politics in Chinese popular music. International Journal of Cultural Studies, Vol. 5, No. 3, 263-290
Go download and read la...
Labels: Faye Wong, Lab Rotation
posted by teopag @ 4:25 PM,
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8th March 2007
Thursday, March 08, 2007
Today finally i got exposed to new laboratory techniques... tissue culture work... It would not be just doing molecular biology all the while, at least i got something new to play on... Even though i have done it during undergraduate time, but it was very long ago... Haha... Tomorrow will do transfection and electroporation... :)
Happy Women's Day!
Happy birdday to Frank, Cheah Wei and Kah Chen... Don't be sam pat... Hahaha...
Night time got earthquake.., but i didn't feel it... :( i was in first floor, i should be feeling it if i were in my room...
Labels: earthquake, Lab Rotation, Laboratory
posted by teopag @ 11:16 PM,
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Cleaning the lab
Thursday, February 15, 2007
Today we cleaned our lab after the lab meeting...
We were cleaning our bench stuffs and organising the public area. It's a good practice to organise and sort out our bench stuffs once a while. Now the whole lab are ready to welcome the CNY... Hahaha...
Labels: Lab Rotation, Laboratory
posted by teopag @ 7:00 PM,
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"End teeth"
Tuesday, February 13, 2007
Today we had out work end lunch at 饗。食天堂...
http://www.eatogether.com
It's a Chinese tradition to have a meal together with our collegues before the Chinese New Year. The place is nice decorated with many variety of food, it just very expensive...
Labels: Lab Rotation, Laboratory, MCB, TIGP
posted by teopag @ 11:49 PM,
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Lab day
Friday, February 09, 2007
Today's lab work was not conducted smoothly.., during the competent cells preparation, i didn't notice that the centrifuge swing rotor couldn't be used to spin large bottles eventhough the slots are fit, my bottles were stucked within the rotor!!! "Big look lo...", luckily CCWang helped me to retrieve the bottles from the swing rotor... Hu... I wasn't planned properly what i want to do today, so being doing everything concurrently making me very tired... Luckily i got the desired clone confirmed by restriction analysis... :)
Labels: Lab Rotation
posted by teopag @ 10:43 PM,
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Strawberry "muah-chee"
Friday, February 02, 2007
Today my labmate Frank gave me one "muah-chee"... It was very nice containing strawberry coated with red bean paste... Thank you!
Labels: Lab Rotation, Laboratory
posted by teopag @ 9:38 PM,
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First day of lab rotation
Monday, January 15, 2007
Today is my first day of lab rotation...
It's considered a "revision" for all those familiar laboratory techniques and research life...
A good start!
Not to forget, Happy birthday to Sook Fun and Phooi Leng!
Labels: Happy Birthday, Lab Rotation, Laboratory, research, Teoh Pak Guan, teopag
posted by teopag @ 10:02 PM,
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