14th April 2007
Saturday, April 14, 2007
Yesterday i carried out the experiment by myself, from in vitro transcription synthesising subgenomic replicon RNA to infect the cells through electroporation. I was a bit chicken hand duck leg (clumsy) during the tissue culture part as i was not confident, so many procedures need to be carried out properly. Finally i made it! So now need to monitor the expression level of reporter gene indicating the replication of introduced RNA in the cells which will eventually contribute to the stable clones establishment. So need to monitor for 5 days to see the replication and two weeks for the stable clones. Orz...
Today is Saturday, but it's a working day...to replace the holiday on last Friday. Good Friday is not a public holiday here, because last Thursday is Qing Ming. Qing Ming (fall on 5th of April every year) is the day for the Chinese to visit the graveyard showing their appreciation and gratitude for the contribution of their ancestors and grandparents. It is a good culture to cultivate the morality among the young generation especially on appreciation and gratefulness of the old generations. It would be a rainy day, and the sky will appeared moody yet clear and bright, that's why it's called Qing (clear) Ming (bright)...
Today got class, because last Friday's class was postponed because of "Good Friday"... Next Saturday is the mid term exam for Cellular and Molecular Immunology. Alamak, semua belum sentuh lagi...
Labels: Lab Rotation, Laboratory, Qing Ming
posted by teopag @ 1:34 AM,
